Chemopreventive effect of celecoxib and expression of cyclooxygenase-2, Casapase-3 and AGNOR on chemically- induced rat submandibular salivary gland neoplasm

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BACKGROUND: Cyclooxygenase-2 (COX)-2 inhibitor (Celecoxib) is a non-steroidal antiinflammatory drug (NSAIDs) and over-expression of COX-2 protein and mRNA has been reported in various cancer tissues. Therefore, it has been suggested that COX-2 is related to carcinogenesis. METHODS: Twenty five albino rats were used .They were divided into 3 groups; group I (normal control) and group II and III which was delivered 4-NQ in the drinking water .Meanwhile group III was given 1500 ppm celecoxib. Submandibular salivary glands were obtained after 32 weeks. Immuno-histochemical staining for COX-2 was performed to determine the COX-2 level and Caspase-3 immuno-expression was done for detection of apoptosis and silver nitrate staining of nucleolar organizer regions (AgNORs) was done for estimating the proliferating cells. The data were analyzed using Student's independent t-test and one-way analysis of variance (ANOVA). RESULTS: The group II and III showed pathological evidence of cancer. COX-2 immuno-staining was stronger in group II than in Group III. Caspase-3 immuno-reaction was statistically highly significant in group III (p<0.05) .Meanwhile proliferation estimated by AgNOR nuclear count was statistically highly significant group II (p<0.05). CONCLUSION: The COX-2 expression was increased in group II (untreated group) than group III. Administration of celecoxib demonstrated the chemo-preventive potential against the carcinogenesis through induction of apoptosis and suppression of tumor growth and proliferation. [Mohamed Zayed. Chemopreventive effect of celecoxib and expression of cyclooxygenase-2, Casapase-3 and AGNOR on chemicallyinduced rat submandibular salivary gland neoplasm. Journal of American Science 2010;6(12):555-363]. (ISSN: 1545-1003). http://www.americanscience.org.

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تاریخ انتشار 2010